DocumentCode :
3198202
Title :
In vitro study on the metabolism of epigallocatechin gallate (EGCG) in mouse´s tissues
Author :
Jian Ge ; Yang Liu ; Meng-xin Wang ; Lin Cui ; Bao-yu Han
Author_Institution :
Dept. of Pharmacy, China Jiliang Univ., Hangzhou, China
Volume :
2
fYear :
2012
fDate :
3-5 Aug. 2012
Firstpage :
833
Lastpage :
836
Abstract :
The study was designed to investigate the characters of metabolism of epigallocatechin gallate (EGCG) in mouse´s tissues, basing on establishing a RP-HPLC method for determination of EGCG. And the results showed that there was a good linearity over the range 0.1-200.0 mg/L (r>;0.999). The recoveries were more than 85.0% in all tissue samples, and the intra- and inter-day coefficients of variation were less than 10.0% in all cases. So the RPHPLC method was readily applied for quantitative analysis of EGCG in rats´ tissue samples. And it was fastest for metabolism of EGCG in plasma. But the metabolism of EGCG in tissue homogenates varied a lot. It was metabolized slowly in liver homogenate, while it was metabolized comparatively fast in small intestine and large intestine homogenates. And the pH value in large intestine played an important role in the metabolism of EGCG. Therefore, basing on the establishment of method for determination of EGCG in rat´s intestine and feces, the characters of metabolism of EGCG were clarified, so as to provide a basic theory for exploring the metabolic fate and develop an advanced preparation of EGCG.
Keywords :
biochemistry; biological tissues; cellular biophysics; liver; pH; EGCG; RP-HPLC method; epigallocatechin gallate; feces; in vitro study; intestine; linearity; liver homogenate; metabolism; mouse tissues; pH value; quantitative analysis; rat tissue; Biochemistry; Intestines; Liver; Nitrogen; Plasmas; Standards; EGCG; Metabolism; Mouse; RP-HPLC;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Information Technology in Medicine and Education (ITME), 2012 International Symposium on
Conference_Location :
Hokodate, Hokkaido
Print_ISBN :
978-1-4673-2109-9
Type :
conf
DOI :
10.1109/ITiME.2012.6291432
Filename :
6291432
Link To Document :
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