Title :
Reproducibility investigations and different experiments with a new type of electronically-conducting-polymer based bipotentiostatic uric acid sensor
Author_Institution :
Dept. of Electron. Tecnol., Budapest Univ. of Technol. & Econ., Hungary
Abstract :
Reproducibility investigations and different experiments with a new type of electronically-conducting-polymer based bipotentiostatic uric acid sensor have been conducted. The sensor was constructed by using dodecyl sulfate doped poly-N-methyl-pyrrole for enzyme immobilization. This polymer film can incorporate the enzyme in one step during electropolymerisation. By the bipotentiostatic measuring method, the two working electrodes (with and without the enzyme) are analogously prepared and polarized and the currents in the two circuits are measured in parallel in time. The layout, which consists of 4 electrode surfaces (W1, W2, reference, counter) was prepared by thick film technology on sintered alumina ceramic substrates. The reproducibility of the characteristics using various electrode materials (Au, Pt), and the influence of the temperature or current density of the polymerisation have been investigated. Different pre-treatments of the working electrodes have been applied. Comparisons of two types of uricase enzymes have been made
Keywords :
biosensors; conducting polymers; current density; electrochemical electrodes; electrochemical sensors; hybrid integrated circuits; polymer films; polymerisation; proteins; thick film circuits; Al2O3; Au; Au electrodes; Pt; Pt electrodes; bipotentiostatic measuring method; circuit currents; conducting-polymer based bipotentiostatic uric acid sensor; dodecyl sulfate doped poly-N-methyl-pyrrole; electrode materials; electrode surfaces; electronically-conducting-polymer based bipotentiostatic uric acid sensor; electropolymerisation; enzyme immobilization; enzyme incorporation; polarized electrodes; polymer film; polymerisation current density; polymerisation temperature; reproducibility; sintered alumina ceramic substrates; thick film technology; uricase enzymes; working electrode pre-treatment; working electrodes; Biochemistry; Ceramics; Counting circuits; Current measurement; Electrodes; Polarization; Polymer films; Reproducibility of results; Thick films; Time measurement;
Conference_Titel :
Electronics Technology: Concurrent Engineering in Electronic Packaging, 2001. 24th International Spring Seminar on
Conference_Location :
Calimanesti-Caciulata
Print_ISBN :
0-7803-7111-9
DOI :
10.1109/ISSE.2001.931077
