Radical scavenging activities of extract and solvent-solvent partition fractions from Dendrobium Sonia “Red Bom” flower

Author

Shafazila, T.S. ; Lee, Pat M. ; Hung, Lee Kong

Author_Institution

Materials Technology Program, Faculty of Applied Sciences, Universiti Technologi MARA (UiTM), Shah Alam, Malaysia

fYear

2010

fDate

5-7 Dec. 2010

Firstpage

762

Lastpage

765

Abstract

Radical scavenging activities of extract and solvent-solvent partition fractions from the orchid flower, Dendrobium Sonia “Red Bom” were evaluated. Flower of Dendrobium Sonia “Red Bom” was extracted with acidified methanol. The residue was redissolved in water which was then subjected to solvent-solvent partition with organic solvents. Crude extract (CEDS) and three different fractions namely hexane layer (HXL), ethyl acetate layer (EAL) and water layer (WL) were evaluated for the total phenolic and anthocyanin contents and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. The DPPH radical scavenging activity was found to be the highest in WL. The direct relationship between the total anthocyanins content with the radical scavenging activity indicates that anthocyanins are the main contributors for the observed radical scavenging activities for CEDS and WL.

Keywords

Analysis of variance; Bills of materials; Color; Compounds; Methanol; Solvents; Temperature measurement; DPPH; Dendrobium; anthocyanins; antioxidant; orchidaceae; radical scavenging activity;

fLanguage

English

Publisher

ieee

Conference_Titel

Science and Social Research (CSSR), 2010 International Conference on

Conference_Location

Kuala Lumpur, Malaysia

Print_ISBN

978-1-4244-8987-9

Type

conf

DOI

10.1109/CSSR.2010.5773886

Filename

5773886