Title :
Micromachined cell fusion device
Author :
Lee, Sang-Wook ; Shim, Dong-Sik ; Kim, Yong-Kweon ; Cha, Hyeon-Chul
Author_Institution :
Sch. of Electr. Eng., Seoul Nat. Univ., South Korea
fDate :
31 Oct-3 Nov 1996
Abstract :
A cell fusion device was designed and fabricated by micromachining technology. The device has 18 pairs of 40 μm thick electrodes. It was designed for one-to-one based fusion. The polymer-molding and electroplating technique was applied for the fabrication of the cell fusion device. Dielectrophoretic force was used for attracting cells and making pearl chains. Electrofusion of two Chinese cabbage cells was successfully achieved by square pulsed electric fields. The electrofusion of cells was accomplished in 10 minutes
Keywords :
biological specimen preparation; cellular biophysics; electrophoresis; electroplating; microelectrodes; micromachining; micromechanical devices; 10 min; 40 micron; Chinese cabbage cells; attracting cells; dielectrophoretic force; electrodes; electrofusion; electroplating; micromachined cell fusion device; one-to-one based fusion; pearl chains; polymer-molding; square pulsed electric fields; Biological materials; Copper; Electrodes; Etching; Fluidic microsystems; Glass; Microfluidics; Plasma applications; Plasma devices; Polyimides;
Conference_Titel :
Engineering in Medicine and Biology Society, 1996. Bridging Disciplines for Biomedicine. Proceedings of the 18th Annual International Conference of the IEEE
Conference_Location :
Amsterdam
Print_ISBN :
0-7803-3811-1
DOI :
10.1109/IEMBS.1996.656942
