Micromachined cell fusion device

Author

Lee, Sang-Wook ; Shim, Dong-Sik ; Kim, Yong-Kweon ; Cha, Hyeon-Chul

Author_Institution

Sch. of Electr. Eng., Seoul Nat. Univ., South Korea

Volume

1

fYear

1996

fDate

31 Oct-3 Nov 1996

Firstpage

258

Abstract

A cell fusion device was designed and fabricated by micromachining technology. The device has 18 pairs of 40 μm thick electrodes. It was designed for one-to-one based fusion. The polymer-molding and electroplating technique was applied for the fabrication of the cell fusion device. Dielectrophoretic force was used for attracting cells and making pearl chains. Electrofusion of two Chinese cabbage cells was successfully achieved by square pulsed electric fields. The electrofusion of cells was accomplished in 10 minutes

Keywords

biological specimen preparation; cellular biophysics; electrophoresis; electroplating; microelectrodes; micromachining; micromechanical devices; 10 min; 40 micron; Chinese cabbage cells; attracting cells; dielectrophoretic force; electrodes; electrofusion; electroplating; micromachined cell fusion device; one-to-one based fusion; pearl chains; polymer-molding; square pulsed electric fields; Biological materials; Copper; Electrodes; Etching; Fluidic microsystems; Glass; Microfluidics; Plasma applications; Plasma devices; Polyimides;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1996. Bridging Disciplines for Biomedicine. Proceedings of the 18th Annual International Conference of the IEEE

Conference_Location

Amsterdam

Print_ISBN

0-7803-3811-1

Type

conf

DOI

10.1109/IEMBS.1996.656942

Filename

656942