Title :
Microfabrication of a flow immunoassay for the detection of aflatoxin
Author :
Rabbany, Sina Y. ; Wong, Eddy Y. ; Sheppard, Norman F., Jr.
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
fDate :
31 Oct-3 Nov 1996
Abstract :
A flow-displacement immunoassay has been implemented by microfabricating a reactor in a silicon wafer. To increase surface area, antibodies immobilized on a porous support matrix have been introduced into the reactor. To characterize the dissociation kinetics of aflatoxin (AFB2) from the affinity-resin loaded reactor, experiments were conducted at flow rates of 0.15, 0.5, 1.0. and 2.0 ml/min. Dissociation curves, measured using the intrinsic fluorescence of AFB 2, showed an exponential decrease in dissociated AFB2 over time. The apparent dissociation rate constants (kd) values were shown to be flow rate dependent. The kd was extrapolated to be 2.30×10-4 sec-1 at infinite flow rate
Keywords :
biosensors; chemical sensors; dissociation; micromachining; microsensors; reaction rate constants; Si; affinity-resin loaded reactor; aflatoxin detection; antigen-antibody binding; apparent dissociation rate constants; dissociation curves; dissociation kinetics; flow rate dependent; flow-displacement immunoassay; immobilized antibodies; immunosensors; infinite flow rate; intrinsic fluorescence; microfabrication; micromachined reactors; porous support matrix; reactor fabrication; silicon wafer; Biomedical engineering; Chemical analysis; Electronic mail; Engineering in Medicine and Biology Society; Fluorescence; Immune system; Inductors; Kinetic theory; Resists; Silicon;
Conference_Titel :
Engineering in Medicine and Biology Society, 1996. Bridging Disciplines for Biomedicine. Proceedings of the 18th Annual International Conference of the IEEE
Conference_Location :
Amsterdam
Print_ISBN :
0-7803-3811-1
DOI :
10.1109/IEMBS.1996.656943
