In-line image plane digital holographic microscopy for biological cells´ observation

Author

Zhou, Hao ; Gu, Jihua ; Chen, Daqing

Author_Institution

Key Lab. of Modern Opt. Eng., Soochow Univ., Suzhou, China

Volume

4

fYear

2011

fDate

29-31 July 2011

Abstract

In-line image plane digital holographic microscopy method is introduced for measurements of the biological cells. After the description of the configuration of system, the experimental results on the USAF resolution target as the calibration object are presented. Biological cells are located in the system and the sizes of them are calculated by the pixel resolution relationship between different reconstruction distances. This method provides microscopy for sample measurements with variable magnifications.

Keywords

biological techniques; biomedical optical imaging; cellular biophysics; holography; image reconstruction; medical image processing; optical microscopy; USAF resolution target; biological cell measurements; biological cell observation; calibration object; in line image plane digital holographic microscopy; pixel resolution relationship; reconstruction distances; Biology; Biomedical optical imaging; Image reconstruction; Image resolution; Microscopy; Optical filters; Optical imaging; biological cells; digital holographic microscopy; magnification;

fLanguage

English

Publisher

ieee

Conference_Titel

Electronics and Optoelectronics (ICEOE), 2011 International Conference on

Conference_Location

Dalian

Print_ISBN

978-1-61284-275-2

Type

conf

DOI

10.1109/ICEOE.2011.6013438

Filename

6013438