Title :
In-line image plane digital holographic microscopy for biological cells´ observation
Author :
Zhou, Hao ; Gu, Jihua ; Chen, Daqing
Author_Institution :
Key Lab. of Modern Opt. Eng., Soochow Univ., Suzhou, China
Abstract :
In-line image plane digital holographic microscopy method is introduced for measurements of the biological cells. After the description of the configuration of system, the experimental results on the USAF resolution target as the calibration object are presented. Biological cells are located in the system and the sizes of them are calculated by the pixel resolution relationship between different reconstruction distances. This method provides microscopy for sample measurements with variable magnifications.
Keywords :
biological techniques; biomedical optical imaging; cellular biophysics; holography; image reconstruction; medical image processing; optical microscopy; USAF resolution target; biological cell measurements; biological cell observation; calibration object; in line image plane digital holographic microscopy; pixel resolution relationship; reconstruction distances; Biology; Biomedical optical imaging; Image reconstruction; Image resolution; Microscopy; Optical filters; Optical imaging; biological cells; digital holographic microscopy; magnification;
Conference_Titel :
Electronics and Optoelectronics (ICEOE), 2011 International Conference on
Conference_Location :
Dalian
Print_ISBN :
978-1-61284-275-2
DOI :
10.1109/ICEOE.2011.6013438
