Title :
Sensing lysozyme rebinding on molecularly imprinted polymers by scanning electrochemical microscopy
Author :
Chen, Hsiao-Chi ; Lin, Shin ; Lin, Hung-Yin ; Chou, Tse-Chuan
Author_Institution :
Dept. of Chem. Eng., Nat. Cheng Kung Univ., Tainan, Taiwan
Abstract :
Molecularly imprinted polymers, artificial antibodies, were synthesized by printing target biomolecules on a monomer mixture and then forming a highly selective and stable polymer. Scanning electrochemical microscopy (SECM) was employed to measure the reduction current between horseradish peroxide (HRP) conjugated lysozyme antibody and ferrocenylmethol (FMA) after different lysozyme concentrations for rebinding were applied on the molecularly imprinted polymers. We found that the reduction current increased linearly with increasing the rebinding lysozyme concentration.
Keywords :
biochemistry; biosensors; electrochemical analysis; enzymes; molecular biophysics; polymers; reduction (chemical); scanning electron microscopy; artificial antibody; biomolecule; ferrocenylmethol; horseradish peroxide; lysozyme concentration; molecularly imprinted polymer; monomer mixture; reduction current; scanning electrochemical microscopy; sensing lysozyme rebinding; Chemical engineering; Cleaning; Current measurement; Glass; Microscopy; Molecular biophysics; Nitrogen; Polymers; Printing; Proteins;
Conference_Titel :
Bioengineering Conference, 2005. Proceedings of the IEEE 31st Annual Northeast
Print_ISBN :
0-7803-9105-5
Electronic_ISBN :
0-7803-9106-3
DOI :
10.1109/NEBC.2005.1431962
