Sensing lysozyme rebinding on molecularly imprinted polymers by scanning electrochemical microscopy

Author

Chen, Hsiao-Chi ; Lin, Shin ; Lin, Hung-Yin ; Chou, Tse-Chuan

Author_Institution

Dept. of Chem. Eng., Nat. Cheng Kung Univ., Tainan, Taiwan

fYear

2005

fDate

2-3 April 2005

Firstpage

138

Lastpage

139

Abstract

Molecularly imprinted polymers, artificial antibodies, were synthesized by printing target biomolecules on a monomer mixture and then forming a highly selective and stable polymer. Scanning electrochemical microscopy (SECM) was employed to measure the reduction current between horseradish peroxide (HRP) conjugated lysozyme antibody and ferrocenylmethol (FMA) after different lysozyme concentrations for rebinding were applied on the molecularly imprinted polymers. We found that the reduction current increased linearly with increasing the rebinding lysozyme concentration.

Keywords

biochemistry; biosensors; electrochemical analysis; enzymes; molecular biophysics; polymers; reduction (chemical); scanning electron microscopy; artificial antibody; biomolecule; ferrocenylmethol; horseradish peroxide; lysozyme concentration; molecularly imprinted polymer; monomer mixture; reduction current; scanning electrochemical microscopy; sensing lysozyme rebinding; Chemical engineering; Cleaning; Current measurement; Glass; Microscopy; Molecular biophysics; Nitrogen; Polymers; Printing; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 2005. Proceedings of the IEEE 31st Annual Northeast

Print_ISBN

0-7803-9105-5

Electronic_ISBN

0-7803-9106-3

Type

conf

DOI

10.1109/NEBC.2005.1431962

Filename

1431962