Title :
Combined SHG signal information with AFM imaging to assess conformational changes in collagen
Author :
Stylianou, A. ; Kyriazi, M. ; Politopoulos, K. ; Yova, D.
Author_Institution :
Lab. of Biomed. Opt. & Appl. Biophys., Nat. Tech. Univ. of Athens, Athens, Greece
Abstract :
Collagen is the most abundant protein in mammals and is important for a variety of functions and its concentration, structure and function is associated with different pathological states. In this research we correlate structural changes with those changes in the Second Harmonic Generation (SHG) signal. The combination of Atomic Force Microscopy (AFM) imaging with information included in SHG signal can significantly contribute to further understanding of the nonlinear optical properties of collagen.
Keywords :
atomic force microscopy; bio-optics; biological techniques; molecular biophysics; optical harmonic generation; proteins; AFM imaging; SHG signal; atomic force microscopy; collagen; conformational changes; mammals; nonlinear optical properties; pathological states; protein; second harmonic generation signal; structural changes; Atom optics; Atomic force microscopy; Nonlinear optics; Optical harmonic generation; Optical imaging; Optical microscopy; Pathology; Proteins; Atomic Force Microscopy; Collagen; Collagen Denaturation; Non linear Optics; Second Harmonic Generation;
Conference_Titel :
Information Technology and Applications in Biomedicine, 2009. ITAB 2009. 9th International Conference on
Conference_Location :
Larnaca
Print_ISBN :
978-1-4244-5379-5
Electronic_ISBN :
978-1-4244-5379-5
DOI :
10.1109/ITAB.2009.5394311
