Title :
Noise reduction in fluorescence Optical Projection Tomography
Author :
Darrell, A. ; Marias, K. ; Brady, M. ; Meyer, H. ; Birk, U. ; Ripoll, J.
Author_Institution :
Inst. of Comput. Sci., Found. for Res. & Technol.-Hellas (FORTH), Heraklion
Abstract :
Optical Projection Tomography (OPT) suffers from additive CCD detector noise. This paper investigates the nature of the CCD detector noise for an OPT setup. The CCD detector noise is found to be described well by an intensity dependent zero-mean Gaussian distribution. A method of reducing the effect of noise in fluorescence OPT reconstructions is discussed. The results of the noise reduction are demonstrated using both artificial scenes created with fluorescent microspheres and real auto-fluorescence OPT projections of the cuticle of the amphipod crustacean Parhyale hawaiensis.
Keywords :
CCD image sensors; Gaussian distribution; bio-optics; cellular biophysics; fluorescence; optical noise; optical projectors; optical tomography; CCD detector noise; Gaussian distribution; Parhyale hawaiensis; fluorescence; microspheres; noise reduction; optical projection tomography; Additive noise; Charge coupled devices; Detectors; Fluorescence; Gaussian distribution; Gaussian noise; Noise reduction; Optical noise; Optimized production technology; Tomography; Fluorescence; Noise; Optical Projection Tomography; Tomography;
Conference_Titel :
Imaging Systems and Techniques, 2008. IST 2008. IEEE International Workshop on
Conference_Location :
Crete
Print_ISBN :
978-1-4244-2496-2
Electronic_ISBN :
978-1-4244-2497-9
DOI :
10.1109/IST.2008.4659940
