DocumentCode :
3232640
Title :
Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates
Author :
Cha, Byung Hak ; Lee, Sang-Myung ; Hwang, Kyo Seon ; Kim, Sang Kyung ; Lee, Yoon-Sik ; Ju, Byeong-Kwon ; Kim, Tae Song
Author_Institution :
Nano Bio Res. Center, Korea Inst. of Sci. & Technol., Seoul
fYear :
2008
fDate :
6-9 Jan. 2008
Firstpage :
160
Lastpage :
163
Abstract :
As the real sample diagnosis using resonance-based microcantilevers, a simple method is strongly suggested that capture probe immobilized on the cantilever directly hybridize with nanoparticles conjugated detection probe. Two different dimensions of microfabricated piezoelectric cantilevers and two different sizes of nanoparticles were used in this experiment. As a result, the most suitable conditions, the 30times90 mum2 (width times length) of the cantilever and the 140 nm of the nanoparticles, were chosen for this method. And quantitative analysis was carried out in different concentrations of nanoparticles conjugated detection probe which are 1 mug/mL, 10 mug/mL and 100 mug/mL. In addition, the sensitivity of this method was demonstrated through the SNP (single nucleotide polymorphism) detection by comparing with quantitative data of hybridization in full matched sequence at same concentration.
Keywords :
DNA; biosensors; cantilevers; microsensors; nanoparticles; piezoelectric devices; DNA detection; SNP detection; microcantilever; nanoparticles conjugates; piezoelectric cantilevers; quantitative analysis; single nucleotide polymorphism detection; Biosensors; Chemical technology; DNA; Force measurement; Nanobioscience; Nanoparticles; Probes; Sequences; Silicon compounds; Stress; Mass enhancement and SNP; Microcantilever;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2008. NEMS 2008. 3rd IEEE International Conference on
Conference_Location :
Sanya
Print_ISBN :
978-1-4244-1907-4
Electronic_ISBN :
978-1-4244-1908-1
Type :
conf
DOI :
10.1109/NEMS.2008.4484309
Filename :
4484309
Link To Document :
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