Statistical analysis of a HPLC method for microcystins determination in drinking water

Author

Ning, Lu ; Jingxian, Sun ; Fanxin, Kong ; Zhang Dong

Author_Institution

Shanghai Municipal Water Resource Dev. & Utilization Nat. Eng. Center Co., Ltd., Shanghai, China

fYear

2011

fDate

22-24 April 2011

Firstpage

4469

Lastpage

4471

Abstract

Statistical analysis of high performance liquid chromatography (HPLC) method for the determination of 0.1~1.0 mg·L^-1 microcystins (MCs) in drinking water was investigated to optimize the instrument operating conditions and reduce the detection limit. The detection limits were obtained by two ways. One through method detection limit (MDL) and the other through detection limit from the calibration curve (DTC). The Cochran test results revealed the homoscedasticity of the HPLC method. The effect of system drift on the calibration curve was noticed by the lack of fit (LOF) test. MDL yielded a less reliable detection limit than DTC. The detection limit of HPLC method for MC-LR was 0.009 mg·L^-1 under recommended operating conditions.

Keywords

chromatography; microorganisms; statistical testing; toxicology; water pollution; water quality; water supply; Cochran test; HPLC method; LOF test; calibration curve; drinking water; high performance liquid chromatography; homoscedasticity; lack of fit test; method detection limit; microcystins determination; statistical analysis; Algae; Calibration; Monitoring; Peptides; Risk management; Toxicology; Water resources; Cochran test; detection limit; lack of fit test; microcystins;

fLanguage

English

Publisher

ieee

Conference_Titel

Electric Technology and Civil Engineering (ICETCE), 2011 International Conference on

Conference_Location

Lushan

Print_ISBN

978-1-4577-0289-1

Type

conf

DOI

10.1109/ICETCE.2011.5775187

Filename

5775187