Title :
Preliminary investigation on an optical nanobiosensor based on Au cap-shaped polystyrene nanospheres
Author :
Cao, Zhong ; Huang, Xi-Xi ; Yan, Feng ; Gong, Fu-Chun ; Li, He-Ping
Author_Institution :
Sch. of Chem. & Environ. Eng., Changsha Univ. of Sci. & Technol., Changsha
Abstract :
An optical biosensor with surface bound gold cap-shaped nano particles like polystyrene nanospheres has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). It caused 1.09 nm red shift in the peak wavelength (lambdamax) of LSPR spectrum for the nanosensor exposed to c-Myc (3C7) mouse monoclonal IgG and 3.53 nm red shift to the corresponding antibody by the immuno -reaction subsequently. The kinetic process for the activity of the c-Myc (3C7) antigen lost with the time has been examined. The LSPR nanobiosensor was also applied to recognition of a DNA plasmid of pCMV-Myc Mammalian Expression Vector, which would provide valuable information on molecular biology in clinical medicine, disease prediction, gene mutation and gene diagnosis.
Keywords :
macromolecules; nanobiotechnology; nanoparticles; surface plasmon resonance; biomolecules detection; localized surface plasmon resonance; mammalian expression vector; nanoparticles; optical nanobiosensor; polystyrene nanospheres; Biomedical optical imaging; Biosensors; DNA; Gold; Mice; Nanobioscience; Optical surface waves; Plasmons; Proteins; Resonance; Au cap-shaped nano particle; Localized surface plasmon resonance; Nanobiosensor; c-Myc;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2008. NEMS 2008. 3rd IEEE International Conference on
Conference_Location :
Sanya
Print_ISBN :
978-1-4244-1907-4
Electronic_ISBN :
978-1-4244-1908-1
DOI :
10.1109/NEMS.2008.4484522