Title :
Application of Femtosecond Laser in Biotechnology: Manipulation and Detection of Living Cells
Author :
Gong, Jixian ; Wu, Zhijun ; Zhao, Xueming ; Wang, Changlei ; Xing, Qirong
Author_Institution :
Dept. of Biochem. Eng., Tianjin Univ., Tianjin, China
Abstract :
Femtosecond laser has been applied to biological research for its advantages of ultra-high temporal and spatial resolutions, such as optical perforation for DNA transfection, ablation of organelle within living cells. The promise shown by femtosecond laser applied to the biological field has led us to propose femtosecond laser for single-cell level research in biotechnology. Femtosecond optical tweezers, femtosecond laser induced cell fusion and detection of intracellular metabolite by THz time-domain spectroscopy were achieved in our research. The result demonstrated that femtosecond laser would give great potential application in biotechnology and bioengineering.
Keywords :
DNA; bio-optics; biochemistry; biotechnology; cellular biophysics; genetics; high-speed optical techniques; laser beam applications; microorganisms; radiation pressure; terahertz wave spectra; time-domain analysis; DNA transfection; THz time-domain spectroscopy; algae cells; bioengineering; biological research; biotechnology; femtosecond laser induced cell fusion; femtosecond optical tweezer; intracellular metabolite detection; living cell detection; living cell manipulation; optical perforation; organelle ablation; single-cell level research; ultra-high spatial resolution; ultra-high temporal resolution; Biomedical optical imaging; Biotechnology; Cells (biology); DNA; Laser ablation; Laser applications; Laser fusion; Spatial resolution; Time domain analysis; Ultrafast optics;
Conference_Titel :
Photonics and Optoelectronics, 2009. SOPO 2009. Symposium on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-4412-0
DOI :
10.1109/SOPO.2009.5230136
