Application of Femtosecond Laser in Biotechnology: Manipulation and Detection of Living Cells

Femtosecond laser has been applied to biological research for its advantages of ultra-high temporal and spatial resolutions, such as optical perforation for DNA transfection, ablation of organelle within living cells. The promise shown by femtosecond laser applied to the biological field has led us to propose femtosecond laser for single-cell level research in biotechnology. Femtosecond optical tweezers, femtosecond laser induced cell fusion and detection of intracellular metabolite by THz time-domain spectroscopy were achieved in our research. The result demonstrated that femtosecond laser would give great potential application in biotechnology and bioengineering.