Adherent cells localization based on Gaussian filtering in automated microinjection system

Author

Ping Gao ; Haibo Huang ; Guodong Chen ; Liguo Chen ; Tao Chen

Author_Institution

Jiangsu Key Lab. for Adv. Robot. Technol., Soochow Univ., Suzhou, China

fYear

2013

fDate

26-30 Aug. 2013

Firstpage

166

Lastpage

169

Abstract

This work is based on the cells in the microinjection system to process adherent cells image. Performing low-pass filtering to the Image of adherent cells, removing uneven illumination, segmenting foreground and background, removing invalid small areas, operating opening and closing operation, keeping the target area, calibrating centers of the cells in the original image.

Keywords

adhesion; biomechanics; biomedical optical imaging; calibration; cellular biophysics; image segmentation; low-pass filters; medical image processing; Gaussian filtering; adherent cell image; adherent cell localization; automated microinjection system; calibrating centers; closing operation; low-pass filtering; operating opening; segmenting background; segmenting foreground; small area removal; Calibration; Cutoff frequency; Filtering; Image segmentation; Lighting; Microinjection; Microscopy; Removal of small areas; Segmentation; cellular localization; filtering;

fLanguage

English

Publisher

ieee

Conference_Titel

Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), 2013 International Conference on

Conference_Location

Suzhou

Print_ISBN

978-1-4799-1210-0

Type

conf

DOI

10.1109/3M-NANO.2013.6737406

Filename

6737406