Biochemical sensors

Author

Heineman, W.R. ; Halsall, H.B.

Author_Institution

Dept. of Chem., Cincinnati Univ., OH, USA

fYear

1992

fDate

22-25 June 1992

Firstpage

102

Lastpage

105

Abstract

Biosensors based on a biological reaction that is very specific for the analyte can achieve a high level of selectivity. Examples are given for sensors based on enzymatic and immunological reactions. An enzymatic-based sensor for lactate has been made by immobilizing lactate oxidase on platinized graphite electrodes. The enzyme is entrapped in a polymeric network of poly(vinyl alcohol) that is formed by gamma radiation cross-linking. Capillary immunoassays that require les than 100 mu L of sample and that can detect extremely small amounts of analyte are examples of sensors based on immunological reactions. A detection limit of only 30000 molecules for IgG in serum was achieved with a sandwich assay using a 70- mu L hematocrit blood collection tube as the capillary.<>

Keywords

biosensors; blood; capillarity; chemical sensors; electrochemical analysis; polymer films; C-Pt; blood collection tube; capillary immunoassays; enzymatic-based sensor; gamma radiation cross-linking; immunological reactions; lactate; organic compounds; poly(vinyl alcohol; polymeric network; Biochemistry; Biosensors; Chemical and biological sensors; Chemical elements; Electrodes; Electrostatic analysis; Gamma ray detectors; Gamma rays; Immune system; Polymer films;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensor and Actuator Workshop, 1992. 5th Technical Digest., IEEE

Conference_Location

Hilton Head Island, SC, USA

Print_ISBN

0-7803-0456-X

Type

conf

DOI

10.1109/SOLSEN.1992.228315

Filename

228315