Title :
Fusarium Oxysporum Growth Inhibition by a Siderophore-Producing Endophytic Bacterial Strain
Author :
Tang Mei ; Zhang Xi-yu ; Yang Ya-zhen ; Liu Fang ; Gong Ming-fu ; Lin Tian-xing ; Guan Qin-lan
Author_Institution :
Key Lab. of Special Biol. Resources in Mountain Emei, Leshan Normal Univ., Leshan, China
Abstract :
In this study, a strain of siderophore-producing endophytic bacterial strain, HS-4, was isolated using improved sugar-aspartic acid (MSA) as a selective medium. The antifungal effect of this bacteria was then assessed against cotton wilt pathogen (Fusarium oxysporum) at varying concentrations of FeCl3. This strain was identified using morphological, biochemical and physiological characteristics, 16S rDNA sequence homology and phylogenetic analysis. Strain HS-4 produced fluorescent siderophores in MSA medium, the relative content of which amounted to 80%. The siderophore produced by strain HS-4 exhibited significant inhibitory activity against F. oxysporum growth under low iron conditions. Strain HS-4 was identified as Bacillus atrophaeus.
Keywords :
biochemistry; biotechnology; cotton; crops; microorganisms; plant diseases; 16S rDNA sequence homology; Bacillus atrophaeus; F. oxysporum growth; FeCl3; FeCl3 concentration; Fusarium oxysporum growth inhibition; HS-4 strain; MSA medium; antifungal effect; biochemical characteristics; cotton wilt pathogen; fluorescent siderophore; inhibitory activity; low iron condition; morphological characteristics; phylogenetic analysis; physiological characteristics; siderophore-producing endophytic bacterial strain; sugar-aspartic acid; Fluorescence; Iron; Liquids; Microorganisms; Production; Solids; Strain; Bacillus atrophaeus; Endophytic bacteria; Fusarium oxysporum; siderophore;
Conference_Titel :
Intelligent System Design and Engineering Applications (ISDEA), 2013 Third International Conference on
Conference_Location :
Hong Kong
Print_ISBN :
978-1-4673-4893-5
DOI :
10.1109/ISDEA.2012.188
