Fabrication of reusable whole PDMS biochip for mesenchymal stem cell separation and enrichment

A novel biochip, which was made from Polydimethylsiloxane (PDMS) and integrated separation and enrichment structures, has been presented. The biochip mainly composes of the spiral microchannel with pillared filtration structures being used to simultaneously and continuously separate cells, and the U-shaped filter being applied to enrich the cells. The protocol of the soft lithographic methods for the whole PDMS biochip is described. The reversible PDMS-PDMS bonding technique is important for the reusable biochip, therefore, the biochip will reuse after separation and enrichment of cells, which can reduce the cost. The simple fabrication processes are attractive for mesenchymal stem cell (MSC) or other cell cultivation, which has potential for clinical application.