Title :
Nanoparticle crystal in tip (NPC-IT): A facile scheme for nanofluidics-based biosensing
Author :
Zhou, Qing ; Wang, Wei ; Wu, Wengang ; Li, Zhihong
Author_Institution :
Sch. of Basic Med. Sci., Peking Univ., Beijing, China
Abstract :
This work introduced a facile scheme for nanofluidics-based biosensing. Streptavidin-modified nanoparticles were packed in a tip to form nanoparticle crystal (NPC-IT). Conductance variation of this streptavidin modified NPC-IT caused by the binding induced surface charge density change was measured to trace-back the target biotin-D concentration. The preliminary experimental results indicated a 10nM sensitivity of biotin detection, which demonstrated the applicability of the present NPC-IT scheme in nanofluidics-based biosensing.
Keywords :
biochemistry; bioelectric phenomena; biosensors; nanobiotechnology; nanofluidics; nanoparticles; nanosensors; proteins; SiO2; binding induced surface charge density; biotin-D concentration; conductance variation; nanofluidics-based biosensing; silica nanoparticles; streptavidin-modified nanoparticles; Biosensors; Crystals; Electrokinetics; Nanofluidics; Nanoparticles; Scanning electron microscopy; Silicon compounds; Nanoparticle crystal; biotin-streptavidin reaction; nanofluidics-based biosensing;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on
Conference_Location :
Kaohsiung
Print_ISBN :
978-1-61284-775-7
DOI :
10.1109/NEMS.2011.6017287
