Title :
THz differential spectroscopy of Rhodopsin
Author :
Vickers, A.J. ; Ma, Y. ; Dudley, R. ; Reeves, P.J. ; Reynolds, C.A. ; Gadde, S.
Author_Institution :
Dept. of Comput. & Electr. Syst., Essex Univ., Colchester
Abstract :
In this work we record THz spectra of Bovine Rhodopsin Rod Outer Segment (ROS) suspended in buffer. THz difference spectra (calculated using spectra collected before and after light excitation) were used to identify signals arising from the domain structural transformations which occur during the light-activation process. The Molecular Modelling Toolkit (MMTK) software package was used to calculate the theoretical normal mode density for both dark-state and light-activated rhodopsin. The simulation results were used to guide the interpretation of spectra observed.
Keywords :
biological techniques; cellular biophysics; molecular biophysics; proteins; terahertz wave spectra; vibrational modes; Molecular Modelling Toolkit software package; bovine rhodopsin rod outer segment; dark-state rhodopsin; domain structural transformations; light-activated rhodopsin; light-activation process; normal mode density; terahertz differential spectroscopy; vibrational modes; Biology computing; Bovine; Frequency; Laboratories; Lighting; Physics computing; Proteins; Retina; Software packages; Spectroscopy;
Conference_Titel :
Infrared, Millimeter and Terahertz Waves, 2008. IRMMW-THz 2008. 33rd International Conference on
Conference_Location :
Pasadena, CA
Print_ISBN :
978-1-4244-2119-0
Electronic_ISBN :
978-1-4244-2120-6
DOI :
10.1109/ICIMW.2008.4665853
