Avian influenza-DNA hybridization detection using wavelength interrogation-based surface plasmon resonance biosensor

Author

Kim, Shin Ae ; Kim, Sung June ; Lee, Sang Hun ; Park, Tai Hyun ; Byun, Kyung Min ; Kim, Sung Guk ; Shuler, Michael L.

Author_Institution

Sch. of Electr. Eng. & Comput. Sci., Seoul Nat. Univ., Seoul, South Korea

fYear

2009

fDate

25-28 Oct. 2009

Firstpage

832

Lastpage

835

Abstract

A wavelength interrogation-based surface plasmon resonance (SPR) biosensor has been investigated for a detection of avian influenza DNA (AI-DNA). The hybridization reaction between AI-DNA and the immobilized oligonucleotide probe on a gold surface was monitored. The results showed that SPR biosensor can provide a linear detection suitable for quantification of AI-DNA hybridization as well as a large dynamic range. The experimental results were consistent well with numerical calculations using rigorous-coupled-wave analysis (RCWA). This study demonstrates the potential of using wavelength-scanning type SPR biosensor in a simple and real-time detection of AI virus.

Keywords

DNA; biosensors; gold; microorganisms; molecular biophysics; surface plasmon resonance; AI virus; Au; DNA hybridization detection; avian influenza; gold surface; immobilized oligonucleotide probe; numerical calculations; rigorous-coupled-wave analysis; surface plasmon resonance biosensor; wavelength interrogation; Biosensors; DNA; Dynamic range; Gold; Influenza; Monitoring; Plasmons; Probes; Resonance; Surface waves;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2009 IEEE

Conference_Location

Christchurch

ISSN

1930-0395

Print_ISBN

978-1-4244-4548-6

Electronic_ISBN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2009.5398267

Filename

5398267