Fabrications of a continuous-flow PCR-chip using dry film resist

In most miniaturized PCR-chip, the sample solution is introduced into a small chamber, and the DNA amplification is performed by thermal cycling. However, chamber heating and cooling rates are relatively low. But, the continuous-flow type PCR-chip involves the transfer of a PCR mixture through a micro-fluidic channel which passes through three different temperature regions. Therefore, it successfully addresses the problems of low heating and cooling rates. In this paper, a soda-lime glass substrate coated with a DFR of the fluid to cycle through different temperature zones was used to form a micro-channel structure and integrated Pt heater continuous-flow PCR.