Title :
3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system
Author :
Wang, Yu-Fu ; Liang, Jen-I ; Lee, Chien-Te ; Yeh, Ming-Long ; Chen, Shean-Jen
Author_Institution :
Inst. of Biomed. Eng., Nat. Cheng Kung Univ., Tainan, Taiwan
Abstract :
In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.
Keywords :
biomedical materials; hydrogels; laser applications in medicine; laser materials processing; two-photon processes; 3D photo-crosslinking pattern scaffold; 3T3 fibroblasts; PEG hydrogel scaffold; UV irradiation; biocompatible 3D Poly(ethylene) glycol; cell adhesion motif; cell application; cell guided migration; cell manipulation; inner fibrin gel; material cytocompatibility; physiological signal recording; two photon laser scanning system; Adhesives; Lasers; Materials; Optical device fabrication; Optical imaging; Photonics; Three dimensional displays; Hydrogel; Photon Cross-linking; Poly(ethylene) glycol (PEG); Two Photon Laser;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on
Conference_Location :
Kaohsiung
Print_ISBN :
978-1-61284-775-7
DOI :
10.1109/NEMS.2011.6017400
