Real-Time Sensing with Single-Pixel Resolved Kinetics Using Room-Temperature Bonded Biofunctional Nanoslits

We demonstrate (1) the compatibility of a bonding method with the use of pre-functionalized surfaces for the fabrication of biofunctional nanoslits and (2) the use of these devices for biofiltering and real-time sensing with single pixel resolved reaction kinetics. Our method relies on the permanent bond between an asymmetrically plasma treated polysilsesquioxane (PSQ) layer and a glass chip occurring at room temperature. Our PSQ-based technique is used for the fabrication of nanoslits which offer reduced diffusion length during fluorescent target-probe reaction. We present hybridization experiments demonstrating that an improved reaction rate, enabling the ultra-fast detection of molecules, can be achieved using such biofunctionalized nanoslits.