Title :
Control single dsDNA molecule stretching and transportation by using virtual nanopore trapper
Author :
Chang, Chia-Jung ; Wang, Pen-Cheng ; Tseng, Fan-Gang
Author_Institution :
Dept. of Eng. & Syst. Sci., Nat. Tsing Hua Univ., Hsinchu, Taiwan
Abstract :
In this paper we demonstrate the adoption of both electrophoresis (EP) and dielectrophoretic (DEP) forces inside a nanoconfinement region to stretch single dsDNA molecule and control the moving speed. By adjusting DEP field inside the nanopore, the opening of the virtual nanopore can be manipulated to drag or trap dsDNA molecule in the virtual nanochannel region. EP force was applied to stretch the dsDNA across the virtual nanopore. The transportation speed of dsDNA molecules can be controlled in a range from 0.615 to 2.285 μm/s, slow enough for DNA sequencing. This virtual nanopore can be used as an electro-tweezers within micro/nano fluidic systems.
Keywords :
DNA; bioMEMS; bioelectric phenomena; biotransport; electrophoresis; microfluidics; molecular biophysics; molecular configurations; nanofluidics; nanoporous materials; DNA sequencing; control single dsDNA molecule stretching; control single dsDNA molecule transportation; dielectrophoretic forces; dsDNA molecule transportation speed; electro-tweezers; electrophoresis; microfluidic system; nanoconfinement region; nanofluidic system; virtual nanochannel region; virtual nanopore trapper; DNA; Electrodes; Etching; Force; Nanobioscience; Nanoscale devices; Silicon; DEP; DNA; sequencing; trapper;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on
Conference_Location :
Kaohsiung
Print_ISBN :
978-1-61284-775-7
DOI :
10.1109/NEMS.2011.6017468
