Control single dsDNA molecule stretching and transportation by using virtual nanopore trapper

Author

Chang, Chia-Jung ; Wang, Pen-Cheng ; Tseng, Fan-Gang

Author_Institution

Dept. of Eng. & Syst. Sci., Nat. Tsing Hua Univ., Hsinchu, Taiwan

fYear

2011

fDate

20-23 Feb. 2011

Firstpage

772

Lastpage

775

Abstract

In this paper we demonstrate the adoption of both electrophoresis (EP) and dielectrophoretic (DEP) forces inside a nanoconfinement region to stretch single dsDNA molecule and control the moving speed. By adjusting DEP field inside the nanopore, the opening of the virtual nanopore can be manipulated to drag or trap dsDNA molecule in the virtual nanochannel region. EP force was applied to stretch the dsDNA across the virtual nanopore. The transportation speed of dsDNA molecules can be controlled in a range from 0.615 to 2.285 μm/s, slow enough for DNA sequencing. This virtual nanopore can be used as an electro-tweezers within micro/nano fluidic systems.

Keywords

DNA; bioMEMS; bioelectric phenomena; biotransport; electrophoresis; microfluidics; molecular biophysics; molecular configurations; nanofluidics; nanoporous materials; DNA sequencing; control single dsDNA molecule stretching; control single dsDNA molecule transportation; dielectrophoretic forces; dsDNA molecule transportation speed; electro-tweezers; electrophoresis; microfluidic system; nanoconfinement region; nanofluidic system; virtual nanochannel region; virtual nanopore trapper; DNA; Electrodes; Etching; Force; Nanobioscience; Nanoscale devices; Silicon; DEP; DNA; sequencing; trapper;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on

Conference_Location

Kaohsiung

Print_ISBN

978-1-61284-775-7

Type

conf

DOI

10.1109/NEMS.2011.6017468

Filename

6017468