Title :
Carbon nanotube based electrochemical immunosensors for high-sensitive detection of E. coli
Author :
Lee, Jun-Yong ; Park, Eun-Jin ; Min, Nam-Ki ; Pak, James Jungho ; Lee, Cheol-Jin ; Kim, Min Ja ; Kim, Soo-Won ; Hong, Suk-In
Author_Institution :
Dept. of Biomicrosystem Technol., Korea Univ., Seoul, South Korea
Abstract :
This paper outlines the fabrication and characterization of an immunosensor based on electrochemical, biological, and nano-material techniques to achieve high sensitive detection of Escherichia coli (E.coli). The working electrode consists of E.coli antibody/antigen/horse-radish peroxidase (HRP) labeled antibody immobilized on plasma-functionalized multiwalled carbon nanotube (pf-MWCNT) film. Immunosensors were characterized by cyclic voltammetry using 10 mM K3Fe(CN)6/3 M KCl and 3, 3´, 5, 5´-tetramethylbenzidine (TMB). This immunosensor shows very high sensitivity to E. coli O157:H7 and detection limit as low as 0.031 ng/ml. This is 100 times lower than that achievable with standard spectrophotometric enzyme-linked immunosorbent assay (ELISA) using the same immunochemicals.
Keywords :
biosensors; carbon nanotubes; electrochemical electrodes; electrochemical sensors; microorganisms; molecular biophysics; nanobiotechnology; nanostructured materials; nanotube devices; plasma materials processing; thin film sensors; voltammetry (chemical analysis); C; E.coli antibody; Escherichia coli; HRP labeled antibody; antigen; biological techniques; carbon nanotube based electrochemical immunosensors; cyclic voltammetry; high sensitivity E. coli detection; horse radish peroxidase; multiwalled carbon nanotube film; nanomaterial techniques; plasma functionalised MWCNT film; working electrode; Argon; Carbon nanotubes; Chemical technology; Electrodes; Ethanol; Fabrication; Humans; Immune system; Intestines; Paper technology;
Conference_Titel :
Sensors, 2009 IEEE
Conference_Location :
Christchurch
Print_ISBN :
978-1-4244-4548-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2009.5398352
