DocumentCode :
3283579
Title :
Electrochemical biosensor for investigation of anticancer drugs interactions (doxorubicin and ellipticine) with DNA
Author :
Trnkova, Libuse ; Stiborova, Marie ; Huska, Dalibor ; Adam, Vojtech ; Kizek, Rene ; Hubalek, Jaromir ; Eckschlager, Tomas
Author_Institution :
Dept. of Chem., Masaryk Univ., Brno, Czech Republic
fYear :
2009
fDate :
25-28 Oct. 2009
Firstpage :
1200
Lastpage :
1203
Abstract :
The mechanisms of action of chemotherapeutics are different, but very often interfere with processes of cell division and can bind onto DNA. The aim of this work was to investigate interactions of antitumour drugs (ellipticine and doxorubicin) with DNA. For these purposes our experiments the screen-printed nanotube carbon electrodes were used. DNA gives oxidation signals for all bases (adenine, cytosine, guanine and thymine). We introduced various ellipticine, doxorubicin and ethidium bromide concentrations to DNA on carbon electrodes fabricated by us and measured the changes in the height of nucleic acid bases signals. In the voltammograms measured the changes associated with the interactions of the target compounds with DNA were evident. The height of DNA signals decreased with increasing ellipticine, doxorubicin and ethidium bromide concentrations. The decrease can be related to changes of DNA structure caused by the intercalation.
Keywords :
DNA; biochemistry; biosensors; cancer; carbon nanotubes; drugs; electrochemical sensors; molecular biophysics; tumours; DNA; adenine; anticancer drugs interactions; antitumour drugs; cell division; chemotherapeutics; cytosine; doxorubicin; electrochemical biosensor; ellipticine; ethidium bromide; guanine; intercalation; screen-printed nanotube carbon electrodes; thymine; voltammograms; Agriculture; Biochemistry; Biosensors; Cancer; Chemistry; DNA; Drugs; Electrodes; Forestry; Tumors;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Sensors, 2009 IEEE
Conference_Location :
Christchurch
ISSN :
1930-0395
Print_ISBN :
978-1-4244-4548-6
Electronic_ISBN :
1930-0395
Type :
conf
DOI :
10.1109/ICSENS.2009.5398361
Filename :
5398361
Link To Document :
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