Title :
Study on DNA electrochemical behavior for label-free micro PCR application
Author :
Zhang, Rong ; Qin, Jian ; Tian, Hao ; Si, Weihua ; Wang, Taihong ; Liu, Zewen
Author_Institution :
Micro-Nano Technol. Res. Center, Hunan Univ., Changsha, China
Abstract :
Electrochemical behaviors of DNA (Deoxyribonucleic acid) are studied for exploring label free PCR (Polymerase Chain Reaction) detection used in miniaturized PCR system. PCR chips with three electrodes are fabricated with CMOS (Complementary Metal Oxide Semiconductor) compatible process. Micro electrodes with different metals successfully are obtained inside the micro PCR reaction cell for optimizing the electrochemical process. Both purified DNA solution diluted in PBS buffer and amplified DsRed DNA solution of n (n=0,4,8,12,20) cycles are used as the solution system. The experiment revealed that the redox peaks current difference between DsRed DNA solution of 4 cycles and 12 cycles is 50 nA.
Keywords :
DNA; biochemistry; biological techniques; electrochemical electrodes; enzymes; lab-on-a-chip; microelectrodes; molecular biophysics; purification; CMOS compatible process; DNA electrochemical behavior; DsRed DNA solution; PBS buffer; PCR chips; complementary metal oxide semiconductor; deoxyribonucleic acid; label-free microPCR application; microPCR reaction cell; microelectrodes; polymerase chain reaction; purified DNA solution; redox peaks; Biosensors; DNA; Educational institutions; Microelectrodes; Optical device fabrication; Optical sensors; DsRed DNA; PCR amplification; PCR product; electrochemical; micro PCR;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on
Conference_Location :
Kaohsiung
Print_ISBN :
978-1-61284-775-7
DOI :
10.1109/NEMS.2011.6017512
