Study on DNA electrochemical behavior for label-free micro PCR application

Author

Zhang, Rong ; Qin, Jian ; Tian, Hao ; Si, Weihua ; Wang, Taihong ; Liu, Zewen

Author_Institution

Micro-Nano Technol. Res. Center, Hunan Univ., Changsha, China

fYear

2011

fDate

20-23 Feb. 2011

Firstpage

952

Lastpage

955

Abstract

Electrochemical behaviors of DNA (Deoxyribonucleic acid) are studied for exploring label free PCR (Polymerase Chain Reaction) detection used in miniaturized PCR system. PCR chips with three electrodes are fabricated with CMOS (Complementary Metal Oxide Semiconductor) compatible process. Micro electrodes with different metals successfully are obtained inside the micro PCR reaction cell for optimizing the electrochemical process. Both purified DNA solution diluted in PBS buffer and amplified DsRed DNA solution of n (n=0,4,8,12,20) cycles are used as the solution system. The experiment revealed that the redox peaks current difference between DsRed DNA solution of 4 cycles and 12 cycles is 50 nA.

Keywords

DNA; biochemistry; biological techniques; electrochemical electrodes; enzymes; lab-on-a-chip; microelectrodes; molecular biophysics; purification; CMOS compatible process; DNA electrochemical behavior; DsRed DNA solution; PBS buffer; PCR chips; complementary metal oxide semiconductor; deoxyribonucleic acid; label-free microPCR application; microPCR reaction cell; microelectrodes; polymerase chain reaction; purified DNA solution; redox peaks; Biosensors; DNA; Educational institutions; Microelectrodes; Optical device fabrication; Optical sensors; DsRed DNA; PCR amplification; PCR product; electrochemical; micro PCR;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on

Conference_Location

Kaohsiung

Print_ISBN

978-1-61284-775-7

Type

conf

DOI

10.1109/NEMS.2011.6017512

Filename

6017512