Title :
A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein
Author :
Noda, Minoru ; Asai, Takeshi ; Yamashita, Kaoru ; Shimanouchi, Toshinori ; Umakoshi, Hiroshi ; Okuyama, Masanori ; Kuboi, Ryouichi
Author_Institution :
Grad. Sch. of Sci. & Technol., Kyoto Inst. of Technol., Kyoto, Japan
Abstract :
We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
Keywords :
bioMEMS; biochemistry; biosensors; electrolytes; leakage currents; micromachining; microsensors; molecular biophysics; proteins; solutions; 1,2-dipalmitoyl-sn-glycero-3-phosphocholline; K4[Fe(CN)6]; Si surface-bulk-micromachining process; biomolecular interaction; carbonic anhydrase from bovine; detectivity; electrolyte-entrapping liposome; leakage current microsensor; liposome-protein interaction; minute liposome solution; specific biomolecule microsensor; Biomembranes; Bovine; Cells (biology); Current measurement; Impedance measurement; Leak detection; Leakage current; Microsensors; Molecular biophysics; Proteins;
Conference_Titel :
Sensors, 2009 IEEE
Conference_Location :
Christchurch
Print_ISBN :
978-1-4244-4548-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2009.5398390
