A nonlinear approach to tracking single nanometer-scale fluorescent particles

Author

Andersson, S.B.

Author_Institution

Dept. of Mech. Eng., Boston Univ., Boston, MA, USA

fYear

2010

fDate

June 30 2010-July 2 2010

Firstpage

4981

Lastpage

4986

Abstract

We describe a nonlinear control law for tracking single, sub-diffraction limit fluorescent particles that takes advantage of the geometric properties of the spatial intensity pattern. We present simulation results for tracking a single particle based on confocal measurements to illustrate the approach. The control law converts each intensity measurement into actuator commands directly, and consequently should yield improved temporal response over existing algorithms. We consider both two- and three-dimensional tracking.

Keywords

biology; fluorescence; geometry; molecular biophysics; nanoparticles; nonlinear control systems; actuator commands; confocal measurements; geometric property; intensity measurement; nonlinear control law; single nanometer-scale fluorescent particle tracking; spatial intensity pattern; subdiffraction limit fluorescent particle; three-dimensional tracking; two-dimensional tracking; Detectors; Fluorescence; Focusing; Image converters; Magnetic field measurement; Microscopy; Nanobioscience; Particle measurements; Particle tracking; Position measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

American Control Conference (ACC), 2010

Conference_Location

Baltimore, MD

ISSN

0743-1619

Print_ISBN

978-1-4244-7426-4

Type

conf

DOI

10.1109/ACC.2010.5530946

Filename

5530946