Title :
Cortical blood flow imaging with a portable MEMS based 2-photon fluorescence microendoscope
Author :
Piyawattanametha, Wibool ; Schnitzer, Mark J.
Author_Institution :
James H. Clark Center for Biomed. Eng. & Sci., Stanford Univ., Stanford, CA, USA
Abstract :
We present a portable microendoscope based on a microelectromechanical systems (MEMS) scanner, compound gradient refractive index micro-lenses and a photonics bandgap fiber (PBF). It overcomes the size (2.0 × 1.9 × 1.1 cm3) and weight (less than 3 grams) limitations of conventional two-photon fluorescence microscopy toward freely moving subjects. The microendoscope utilizes a photonic bandgap fiber for laser excitation and large core fiber for fluorescence collection. We demonstrated cortical blood flow imaging in live mice with transverse (Δx) and axial resolutions (Δz) of 1.6 μm and 13.5 μm, respectively.
Keywords :
bioMEMS; biomedical optical imaging; endoscopes; fluorescence; haemodynamics; haemorheology; micromechanical devices; neurophysiology; refractive index; compound gradient refractive index microlenses; cortical blood flow imaging; fluorescence collection; large core fiber; laser excitation; live mice; microelectromechanical system scanner; photonics bandgap fiber; portable MEMS based 2-photon fluorescence microendoscope; Fluorescence; Image resolution; Imaging; Laser beams; Lenses; Micromechanical devices; Mirrors; 2-photon; MEMS; fluorescence; scanner;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on
Conference_Location :
Kaohsiung
Print_ISBN :
978-1-61284-775-7
DOI :
10.1109/NEMS.2011.6017587
