Cortical blood flow imaging with a portable MEMS based 2-photon fluorescence microendoscope

Author

Piyawattanametha, Wibool ; Schnitzer, Mark J.

Author_Institution

James H. Clark Center for Biomed. Eng. & Sci., Stanford Univ., Stanford, CA, USA

fYear

2011

fDate

20-23 Feb. 2011

Firstpage

1262

Lastpage

1266

Abstract

We present a portable microendoscope based on a microelectromechanical systems (MEMS) scanner, compound gradient refractive index micro-lenses and a photonics bandgap fiber (PBF). It overcomes the size (2.0 × 1.9 × 1.1 cm³) and weight (less than 3 grams) limitations of conventional two-photon fluorescence microscopy toward freely moving subjects. The microendoscope utilizes a photonic bandgap fiber for laser excitation and large core fiber for fluorescence collection. We demonstrated cortical blood flow imaging in live mice with transverse (Δx) and axial resolutions (Δz) of 1.6 μm and 13.5 μm, respectively.

Keywords

bioMEMS; biomedical optical imaging; endoscopes; fluorescence; haemodynamics; haemorheology; micromechanical devices; neurophysiology; refractive index; compound gradient refractive index microlenses; cortical blood flow imaging; fluorescence collection; large core fiber; laser excitation; live mice; microelectromechanical system scanner; photonics bandgap fiber; portable MEMS based 2-photon fluorescence microendoscope; Fluorescence; Image resolution; Imaging; Laser beams; Lenses; Micromechanical devices; Mirrors; 2-photon; MEMS; fluorescence; scanner;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on

Conference_Location

Kaohsiung

Print_ISBN

978-1-61284-775-7

Type

conf

DOI

10.1109/NEMS.2011.6017587

Filename

6017587