Title :
Confocal fluorescence spectral imaging technique and its applications to drug development
Author_Institution :
Shemyakin-Ovchinnikov Inst. of Bioorganic Chem., Moscow, Russia
Abstract :
A spectral analysis that is widely used to study molecular interactions in solutions can be transferred to cellular and tissue levels and is a basis of a confocal spectral imaging (CSI) technique. The technique is a potent tool to deconvolve overlapping spectra of fluorophores in a specimen, to discriminate weak signals of fluorophores interfering with cellular autofluorescence and to study molecular interactions of drugs within living cells. The CSI technique measures a two-dimensional set of spectra with a three-dimensional spatial resolution from a tissue section or an intact living cell treated with a fluorescent drug and analyzes it in order to: identify and map molecular interactions of the drug; quantify accumulation, localization and retention of the drug in the specimen. Features of the CSI technique are illustrated with our data obtained in the course of development and studies of advanced agents for photodynamic and boron neutron-capture anticancer therapies.
Keywords :
biological tissues; biomedical optical imaging; cancer; cellular biophysics; drugs; fluorescence; molecular biophysics; neutron capture therapy; photodynamic therapy; spectral analysis; CSI technique; boron neutron-capture anticancer therapy; cellular autofluorescence; cellular levels; confocal fluorescence spectral imaging technique; drug development; fluorescent drug; fluorophores; intact living cell; living cells; molecular interactions; photodynamic therapy; spectral analysis; three-dimensional spatial resolution; tissue levels; tissue section; Boron; Cancer; Drugs; Fluorescence; Imaging; Neutron capture therapy; Statistical analysis; anticancer; confocal; fluorescence; neutronsensitizer; photosensitizer; spectral imaging;
Conference_Titel :
Photonics Global Conference (PGC), 2012
Conference_Location :
Singapore
Print_ISBN :
978-1-4673-2513-4
DOI :
10.1109/PGC.2012.6457865