Design of a bioreactor to cyclically strain tissue engineered blood vessel rings

Author

Adams, K. ; Bishop, K. ; Casey, E. ; Gwyther, T. ; Rolle, M.

Author_Institution

Dept. of Biomed. Eng., Worcester Polytech. Inst., Worcester, MA, USA

fYear

2011

fDate

1-3 April 2011

Firstpage

1

Lastpage

2

Abstract

Cyclic mechanical loading improves strength and extracellular matrix (ECM) synthesis in tissue engineered blood vessels (TEBV). The goal of this project was to design a device to impart cyclic circumferential stretch on cell-derived TEBV rings cultured on flexible silicone tubing. During static inflation tests, the tubing diameter increased by 10±1.6%. Our device cyclically displaces water (75 μl volume) to inflate tubing at a frequency of 1 Hz. TEBV rings were loaded onto silicone tubing and subjected to mechanical conditioning for 3-7 days. Structural analysis of conditioned TEBV ring samples (compared to statically cultured control rings) is in progress.

Keywords

biomechanics; biomedical equipment; bioreactors; blood vessels; cellular biophysics; internal stresses; mechanical testing; silicones; tissue engineering; bioreactor; cell culture; cell-derived TEBV rings; cultured control rings; cyclic circumferential stretch; cyclic mechanical loading; extracellular matrix synthesis; flexible silicone tubing; frequency 1 Hz; inflation; silicone tubing; static inflation tests; structural analysis; time 3 day to 7 day; tissue engineered blood vessels; Blood vessels; Electron tubes; Electronic countermeasures; Fitting; Loading; Media;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2011 IEEE 37th Annual Northeast

Conference_Location

Troy, NY

ISSN

2160-7001

Print_ISBN

978-1-61284-827-3

Type

conf

DOI

10.1109/NEBC.2011.5778530

Filename

5778530