Title :
Tracking multiple fluorescent particles in two dimensions in a confocal microscope
Author :
Shen, Zhaolong ; Andersson, Sean B.
Author_Institution :
Dept. of Mech. Eng., Boston Univ., Boston, MA, USA
Abstract :
In this paper, we describe a system for tracking multiple fluorescent particles in a confocal microscope. The tracking algorithm relies on position estimates derived from fluorescence measurements taken at a small number of discrete locations combined with an LQG controller. We report experimental results of tracking a single fixed particle, a single diffusing particle, and two fixed particles.
Keywords :
linear quadratic Gaussian control; microscopes; physical instrumentation control; tracking; LQG controller; confocal microscope; discrete locations; fluorescence measurements; multiple tracking fluorescent particles; Biomedical optical imaging; Cells (biology); Fluorescence; Focusing; Laser feedback; Optical microscopy; Particle measurements; Particle tracking; Position measurement; Quantum dot lasers;
Conference_Titel :
Decision and Control, 2009 held jointly with the 2009 28th Chinese Control Conference. CDC/CCC 2009. Proceedings of the 48th IEEE Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-3871-6
Electronic_ISBN :
0191-2216
DOI :
10.1109/CDC.2009.5399752
