Title :
Reprogramming of cells using modified mRNA
Author :
Pandit, V. ; Nesbitt, R.S. ; Macione, J. ; Kotha, S.P.
Author_Institution :
Rensselaer Polytech. Inst., Troy, NY, USA
Abstract :
Reprogramming of cells for delivery of proteins at specific sites holds great promise for future clinical applications. Chitosan delivery agents in form of nano-spheres presents a readily available biodegradable option for controlled release of mRNA in the cytosol of the cells. In this work we are using Enhanced Green Fluorescent protein as a proof of concept to show for transfection of osteoblast cells with modified mRNA. Transfection was carried out using lipofectaime RNAiMAX.
Keywords :
biological techniques; biology computing; cellular biophysics; fluorescence; proteins; biodegradable; cell reprogramming; chitosan delivery agents; enhanced green fluorescent protein; lipofectaime RNAiMAX; modified mRNA; osteoblast cell transfection; proteins; Biomedical imaging; Fluorescence; Immune system; Media; Polymers; Proteins; Stem cells;
Conference_Titel :
Bioengineering Conference (NEBEC), 2011 IEEE 37th Annual Northeast
Conference_Location :
Troy, NY
Print_ISBN :
978-1-61284-827-3
DOI :
10.1109/NEBC.2011.5778580
