Title :
Nanoengineered uric acid biosensor based on chemiluminescence
Author :
Chaudhari, R.D. ; Joshi, A.B. ; Srivastava, Rajesh
Author_Institution :
Dept. of Biosci. & Bioeng., IIT Bombay, Mumbai, India
Abstract :
Uric acid (UA) is a nitrogenous end product of the purine catabolism in humans. Excessive production of UA may lead to gout, hyperuricemia and kidney disorders. The research here aims at developing an indirect enzyme based UA biosensor where UA is sensed from the amount of hydrogen peroxide (H2O2) produced. Pentacene loaded nanoparticles encapsulated in uricase loaded alginate microspheres (nano-in-micro) particles were synthesized. All formulations were characterized using DLS and imaged using Optical, SEM, TEM, CLSM and FTIR. These nano-in-micro particles were used for H2O2 and UA sensing. UA sensing with nano-in-micro system showed regression constant 0.977 in range of 0-0.1mg/ml. Thus chemiluminescent nano-in-micro particles can act as diagnostic markers for detection of UA.
Keywords :
biosensors; chemiluminescence; enzymes; hydrogen compounds; molecular biophysics; nanomedicine; scanning electron microscopy; transmission electron microscopy; FTIR; H2O2; SEM; TEM; chemiluminescence; chemiluminescent microparticle; chemiluminescent nanoparticle; diagnostic markers; enzyme based UA biosensor; hydrogen peroxide; nanoengineered uric acid biosensor; nanoparticle; pentacene loaded nanoparticles; uricase loaded alginate microspheres; Biochemistry; Biosensors; Fluorescence; Nanoparticles; Pentacene; Suspensions; Uric acid; alginate; biosensor; pentacene;
Conference_Titel :
Bioengineering Conference (NEBEC), 2011 IEEE 37th Annual Northeast
Conference_Location :
Troy, NY
Print_ISBN :
978-1-61284-827-3
DOI :
10.1109/NEBC.2011.5778591
