Aligned, electrospun topography for use with external stimulus systems

Variations in fiber density, diameter, and orientation can be used to direct cell migration and guide neurite growth. The characterization of electrospun fibers is essential prior to conducting further research that utilizes electrospun scaffolds to control the magnitude and direction of cell migration and neurite growth. The goal of this study is to create highly aligned, electrospun fibers on poly-L-lactic acid (PLLA) films in parallel and perpendicular orientations to the long axis of rectangular cover slips. Fibers were electrospun into scaffolds and scanning electron microscopy (SEM) was used to characterize fiber density and orientation. The construction of electrospun scaffolds with aligned fibers is important in guiding cell behavior.