Title :
Comparison of heparin-immobilized vs. antibody-immobilized microspheres for the capture and detection of cytokines during microdialysis sampling
Author :
Duo, Jia ; Cabrera, Randy F Espinal ; Stenken, Julie A.
Author_Institution :
Dept. of Chem. & Chem. Biol., Rensselaer Polytech. Inst., Troy, NY
Abstract :
Heparin-immobilized beads were compared against antibody-immobilized beads for improving the collection of targeted cytokines through microdialysis sampling probes. Heparin offers an advantage over antibodies with respect to its stability and cost. The cytokines, MCP-1 and RANTES, exhibited increased flux through the probes when either heparin-immobilized or antibody-immobilized beads were included in the perfusion fluid. This suggests that heparin-immobilized beads may be a viable alternative to antibody-immobilized beads to increase the flux of select cytokines during microdialysis sampling.
Keywords :
biosensors; molecular biophysics; MCP-1 cytokine; RANTES cytokine; antibody immobilized microspheres; heparin immobilized microspheres; microdialysis sampling; Bovine; Buffer storage; Chemicals; Chemistry; Costs; Immune system; Probes; Sampling methods; Stability; Temperature;
Conference_Titel :
Life Science Systems and Applications Workshop, 2009. LiSSA 2009. IEEE/NIH
Conference_Location :
Bethesda, MD
Print_ISBN :
978-1-4244-4292-8
Electronic_ISBN :
978-1-4244-4293-5
DOI :
10.1109/LISSA.2009.4906722
