Title :
Novel hepatocyte encapsulation enhances cellular functions
Author :
Ser-Mien Chia ; Li, Jun ; Xu, Xi ; Gao, Shujun ; Leong, Kam W. ; Yu, Hanry
Author_Institution :
Med. Inst., Nat. Univ. of Singapore, Singapore
Abstract :
We have synthesized a HEMA, MMA and MAA terpolymer to complex with modified collagen for encapsulation of rat hepatocytes. The encapsulated hepatocytes exhibited high level of functions in culture comparable to those of hepatocyte spheroids. The collagen that lined the inner layer of the capsule provided a compatible substrate for hepatocyte culture, and the outer terpolymer shell determined permeability, which was optimized for transport of 60 kDa but not 150 kDa molecules. Besides shorter processing time than spheroid formation, the encapsulation may offer other advantages important in designing a bioartificial liver-assisted device
Keywords :
biological specimen preparation; biomedical materials; cellular transport; encapsulation; liver; polymer blends; proteins; bioartificial liver-assisted device; compatible substrate; complex with modified collagen; enhanced cellular function; hepatocyte culture; hepatocyte encapsulation; rat hepatocytes; semi-permeability; terpolymer synthesis; Biological materials; Biomedical engineering; Biomedical materials; Bioreactors; Cyclic redundancy check; Encapsulation; Optical fiber devices; Permeability; Polymers; Stability;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.803877
