Capillary gel electrophoresis on a disposable PDMS (polydimethysikoxane) microchip

Author

Hong, Jong Wmk ; Hosokawa, Kazuo ; Fujii, L. ; Seki, Minoru ; Endo, Isao

Author_Institution

Dept. of Chem. & Biotechnol., Tokyo Univ., Japan

Volume

2

fYear

1999

fDate

36434

Abstract

A microfabricated polymer (PDMS: polydimethylsiloxane)- based microchip for capillary gel electrophoresis is presented. The chip can be easily produced by a simple and inexpensive molding and sealing method in low cost. The capillary channel on the chip was partially filled with agarose gel that can enhance separation resolution and can shorten channel length required for separation. The chip can be used as an disposable apparatus for biomedical analysis. DNA molecules were successfully driven by electric field and separated to form bands on agarose gel-filled PDMS microchip

Keywords

DNA; biochemistry; biomedical electronics; biomedical measurement; electrophoresis; gels; polymers; PDMS; agarose gel; capillary channel; capillary gel electrophoresis; channel length shortening; disposable polydimethylsiloxane microchip; microfabricated polymer; separation resolution enhancement; Chemical engineering; Costs; DNA; Electrokinetics; Etching; Mechanical engineering; Microchannel; Silicon; Surface morphology; Surface treatment;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.803883

Filename

803883