A kinetic study on fiber optic immunosensor for the detection of protein C

An optical fiber-based fluorosensor utilizing an evanescent wave has been developed for the detection of protein C (PC) and the kinetic response of the sensor was investigated The signal acquisition relied on evanescent field excitation of fluorescence through a tapered optical fiber on which a sandwich immunoassay occurs. A reasonably short and constant measurement time (5 min) for pure PC samples was determined in order to provide consistent and accurate real-time analysis. In testing diluted human serum albumin (HSA) mixtures with PC, there were significant deviations from the pure PC samples as the HSA concentrations increased. The study on the influence of mass transfer limitation was performed and culminated in the optimized reaction time and sample flowrate through the fiber for the efficient testing