Title :
Real-time, multi-wavelength microbe detection instrument
Author :
Estes, C. ; Duncan, A. ; Wade, B. ; Powers, L.
Author_Institution :
Dept. of Electr. & Comput. Eng., Utah State Univ., Logan, UT, USA
Abstract :
A hand-held instrument has been developed for microbial detection on surfaces and in water. Based on intrinsic microbial fluorescent markers, the instrument detects the presence of ~10 cells in ~2 cm2 interrogation area on a surface, such as meats/poultry, surgical tools and other sterile surfaces, and in water. Contact with the sample is not necessary for detection. Six intrinsic fluorescent markers are used simultaneously to determine the presence, quantity, and metabolic state (live/dead) of the microbial contamination. Detection requires seconds
Keywords :
biomedical equipment; biosensors; cellular biophysics; fluorescence; food processing industry; microorganisms; real-time systems; surface contamination; surgery; 2 cm; hand-held instrument; interrogation area; intrinsic fluorescent markers; intrinsic microbial fluorescent markers; meats; metabolic state; microbial contamination; poultry; quantity; real-time multi-wavelength microbe detection instrument; spiked meat samples; sterile surfaces; surfaces; surgical tools; water; Diseases; Fluorescence; Optical design; Optical filters; Optical sensors; Photoelectricity; Surface contamination; Surgery; Surgical instruments; Water pollution;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.804008