Real-time, multi-wavelength microbe detection instrument

Author

Estes, C. ; Duncan, A. ; Wade, B. ; Powers, L.

Author_Institution

Dept. of Electr. & Comput. Eng., Utah State Univ., Logan, UT, USA

Volume

2

fYear

1999

fDate

36434

Abstract

A hand-held instrument has been developed for microbial detection on surfaces and in water. Based on intrinsic microbial fluorescent markers, the instrument detects the presence of ~10 cells in ~2 cm² interrogation area on a surface, such as meats/poultry, surgical tools and other sterile surfaces, and in water. Contact with the sample is not necessary for detection. Six intrinsic fluorescent markers are used simultaneously to determine the presence, quantity, and metabolic state (live/dead) of the microbial contamination. Detection requires seconds

Keywords

biomedical equipment; biosensors; cellular biophysics; fluorescence; food processing industry; microorganisms; real-time systems; surface contamination; surgery; 2 cm; hand-held instrument; interrogation area; intrinsic fluorescent markers; intrinsic microbial fluorescent markers; meats; metabolic state; microbial contamination; poultry; quantity; real-time multi-wavelength microbe detection instrument; spiked meat samples; sterile surfaces; surfaces; surgical tools; water; Diseases; Fluorescence; Optical design; Optical filters; Optical sensors; Photoelectricity; Surface contamination; Surgery; Surgical instruments; Water pollution;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.804008

Filename

804008