Title :
Integrated platform for ion channel sensing
Author :
Wilk, S.J. ; Petrossian, L. ; Goryll, M. ; Thornton, T.J. ; Goodnick, S.M. ; Tang, J.M. ; Eisenberg, R.S.
Author_Institution :
Center for Solid State Electron. Res., Arizona State Univ., Tempe, AZ
fDate :
Oct. 30 2005-Nov. 3 2005
Abstract :
Conductance measurement of single ion channels and related stochastic signals is a promising technique for the development of a functional biosensor. We present results showing that silicon substrates can be used as a low noise, universal platform for recording the electrical activity of single ion channels inserted into bilayer membranes. Bilayers were suspended on polytetrafluoroethylene coated, 150mum apertures etched into silicon substrates and then oxidized with common silicon processing techniques. The overall noise of the system was reduced with a 75mum thick SU-8 layer and integrated silver/silver chloride electrodes. Phase sensitive detection of single OmpF porin ion channels using the silicon device and a lock-in amplifier were made and demonstrate the system´s capability as a sensor in high noise environments
Keywords :
bioelectric phenomena; biomembrane transport; biosensors; electric admittance measurement; polymer films; silicon; 150 micron; 75 micron; SU-8 layer; bilayer membrane; biosensors; conductance measurement; electrical activity; integrated platform; integrated silver-silver chloride electrode; ion channel sensor; lock-in amplifier; overall noise of the system; phase sensitive detection; polytetrafluoroethylene coating; silicon substrate; single OmpF porin ion channel; single ion channel; stochastic signal; Apertures; Biomembranes; Biosensors; Electrodes; Etching; Noise reduction; Silicon; Silver; Stochastic resonance; Working environment noise;
Conference_Titel :
Sensors, 2005 IEEE
Conference_Location :
Irvine, CA
Print_ISBN :
0-7803-9056-3
DOI :
10.1109/ICSENS.2005.1597912
