Title :
A real-time Linux based laser trapping system
Author :
Gaffney, Donald P. ; Kennedy, Guy G. ; Warshaw, David M.
Author_Institution :
Dept. of Molecular Physiol. & Biophys., Vermont Univ., Burlington, VT, USA
Abstract :
A laser trapping system for the study of actomyosin interactions at the single molecule level has been developed using Real-Time Linux (RT-Linux). The laser trap has been successfully employed to examine both displacements and near-isometric forces generated by a single myosin molecular motor interacting with an actin filament. In these assays an actin filament is strung between two beads that may be manipulated in solution by infrared laser traps. It is concluded that RT-Linux has proven to be capable of providing the required timing control as well as accommodating control of other components and data acquisition
Keywords :
biological techniques; measurement by laser beam; molecular biophysics; proteins; radiation pressure; actomyosin interactions; biophysical research technique; data acquisition; infrared laser traps; near-isometric forces; real-time Linux based laser trapping system; timing control; Linux; Real time systems;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.804030
