Title :
A novel hypothesis-testing technique for resolving overlapping peaks in DNA sequencing
Author :
Yuwaraj, M. ; Eizenman, M. ; Davies, S. ; Pasupathy, S.
Author_Institution :
Inst. of Biomed. Eng., Toronto Univ., Ont., Canada
Abstract :
The accuracy of determining the Deoxyribo Nucleic Acid (DNA) sequence is limited by overlapping peaks of the same base-type (A, C, G or T). Commonly used iterative method to resolve these peaks tends to generate false peaks and has limited resolution. This paper presents a novel hypothesis-testing method, which uses a statistical model of DNA time series, to estimate position and amplitude of the overlapping peaks. The new method has better resolution than the iterative method and does not generate false peaks
Keywords :
DNA; biology computing; genetics; maximum likelihood sequence estimation; molecular biophysics; probability; time series; DNA sequencing; DNA time series; Deoxyribo Nucleic Acid; hypothesis-testing technique; overlapping peak amplitude; overlapping peak position; overlapping peak resolution; same base-type; statistical model; Biomedical engineering; DNA; Deconvolution; Genetics; Iterative methods; Pathogens; Probability; Sequences; Signal resolution; Testing;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.804163
