Title :
Thermal therapy in the Dunning AT-1 prostate tumor using a wet electrode RF probe
Author :
Bhowmick, S. ; Lulloff, L. ; Swanlund, D.J. ; Coad, J.E. ; Hoey, M.F. ; Bischof, J.C.
Author_Institution :
Dept. of Mech. Eng., Minnesota Univ., Minneapolis, MN, USA
Abstract :
The effect of supraphysiological thermal therapy on tissue viability was investigated in an AT-1 Dunning rat prostate tumor model. After a thermal protocol was performed with concurrent temperature measurements, an in vivo-in vitro assay was used to assess cellular survival based on known thermal history and tetrazolium dye (TTC) and histology was used to evaluate overall tissue response 1, 3 and 7 days post ablation. The cellular study showed that survival gradually dropped to 0 with an increase in predicted injury (R) from 0.1 to 1. TTC analysis showed significant drop in enzyme activity and morphometry of histology revealed a significant increase in coagulative necrosis (p<0.01 for both studies) after each of 1, 3 and 7 days compared to sham tissue. These results show that controlled thermal destruction can be obtained in AT-1 prostate tumor using thermal therapy with no cellular survival at temperatures >47°C for times ⩾a minute
Keywords :
biological organs; cellular effects of radiation; coagulation; hyperthermia; radiofrequency heating; tumours; Dunning AT-1 prostate tumor; cellular survival; coagulative necrosis; concurrent temperature measurements; controlled thermal destruction; enzyme activity; histology; in vivo-in vitro assay; morphometry; overall tissue response; predicted injury; rat prostate tumor model; tetrazolium dye; thermal therapy; tissue viability; wet electrode RF probe; Biomedical electrodes; History; Injuries; Medical treatment; Neoplasms; Probes; Protocols; Radio frequency; Temperature control; Tumors;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.804451