Application of Reversal Operation in Optimizing Synthesis Strategy for DNA Microarrays

DNA microarrays have become one of the most powerful tools in modern biology. Expanding demand for mass production of microarrays has created considerable interest in improving the cost effectiveness of microarray fabrication techniques. Several algorithms dedicated to reducing the number of synthesis cycles for the in situ synthesis of high density DNA microarrays were presented in this paper. Meanwhile, an operation that reverses the probes on a microarray based on those algorithms was introduced and evaluated. The results indicate a possibility for a further reduction of the synthesis cycles.