DocumentCode
3317987
Title
Notice of Retraction
Research regarding Preparation and Optimization of Preservation Condition of 3alpha Hydroxysteroid Dehydrogenase
Author
Jingjing Fu ; Juankun Zhang ; Ting Fan ; Binbin Zhang ; Min Li
Author_Institution
Tianjin Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
5
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Purpose: To optimize expression and purification conditions of 3α Hydroxysteroid Dehydrogenase (3α-HSD) to prepare highly pure and active interest protein; Methods: To find optimal expression conditions according to the optimization of expression conditions; to find optimal purification conditions according to comparison between the effects of different purification conditions; to obtain optimal preservation of enzyme according to comparison between the effects of different conditions on enzymic activity. Results: The induction expression of protein reaches the peak after 6 hours; the optimization of purification conditions shows that it is the best purification method on economic effects to combine ammonium sulfate precipitation and ion exchange; 3α-HSD is stable under the conditions of less than 25 degree and pH9. Conclusions : To find optimal preparation conditions for 3α-HSD and obtain optimized preservation conditions for enzyme according to optimization of conditions of expression and purification.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Purpose: To optimize expression and purification conditions of 3α Hydroxysteroid Dehydrogenase (3α-HSD) to prepare highly pure and active interest protein; Methods: To find optimal expression conditions according to the optimization of expression conditions; to find optimal purification conditions according to comparison between the effects of different purification conditions; to obtain optimal preservation of enzyme according to comparison between the effects of different conditions on enzymic activity. Results: The induction expression of protein reaches the peak after 6 hours; the optimization of purification conditions shows that it is the best purification method on economic effects to combine ammonium sulfate precipitation and ion exchange; 3α-HSD is stable under the conditions of less than 25 degree and pH9. Conclusions : To find optimal preparation conditions for 3α-HSD and obtain optimized preservation conditions for enzyme according to optimization of conditions of expression and purification.
Keywords
biochemistry; enzymes; ion exchange; precipitation; proteomics; 3α hydroxysteroid dehydrogenase; 3α-HSD; ammonium sulfate precipitation; enzyme; induction expression; ion exchange; preparation conditions; protein; purification; Companies; Damping; Fluids; Optimization; Proteins; Purification;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780037
Filename
5780037
Link To Document