Notice of RetractionProkaryotic Expression and Analysis on the 22kDa Protein Gene of Mycobacterium paratuberculosis

Notice of Retraction

After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.

We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.

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The gene encoding 22kDa protein gene from Mycobacterium paratuberculosis C-2, chromosomal DNA was amplified by using polymerase chain reaction (PCR), the PCR product was approximately 720bp DNA segment. The PCR product was cloned into pMD-18-T vector and the cloning plasmid pMD-18-T-22 was constructed successfully. The purified 22kDa protein gene was subcloned into the expression vector pET32a(+), and the prokaryotic expression Plasmid pET32a-22 was constructed. Plasmid containing pET32a-22 was transformed into competence Escherichia coli BL21 (DE3). The bacterium was induced by isopropyl-β-Dthiogalactopyranoside (IPTG) and its lysates were loaded directly onto sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), approximately 41kDa exogenous protein was observed on the SDS-PAGE. The protein was analyzed by using Western-blotting. The results indicated that the protein was of antigenic reactivity of M. paratuberculosis. The results were expected to lay foundation for further studies on the subunit vaccine, DNA vaccine and diagnostic reagents of 22kDa protein gene in their prevention against bovine paratuberculosis.