Title :
Comprehensive Proteome Analysis of Human Smooth Muscle Cells by 2D-HPLC-ESI-MS/MS
Author :
Zhang, Yongqian ; Lai, Chengjun ; Zhu, Yong ; Deng, Yulin
Author_Institution :
Sch. of Life Sci., Beijing Inst. of Lechnology, Beijing, China
Abstract :
The proteome of smooth muscle cells of human umbilical cord by offline SCX and LC-ESI-MS/MS are investigated. The MS/MS data generated by data-dependent acquisition via the ion trap was searched by the Mascot. 383 proteins were identified and characterized based on annotated component, function, and biological process by Gene Ontology (GO).
Keywords :
cellular biophysics; ion exchange; mass spectroscopy; molecular biophysics; muscle; ontologies (artificial intelligence); proteins; proteomics; 2D-HPLC-ESI-MS; annotated component; biological process; cation exchange fractionation; comprehensive proteome analysis; data-dependent acquisition; gene ontology; human smooth muscle cells; human umbilical cord; ion trap; mass spectrometry; proteins; Biomembranes; Humans; Muscles; Peptides; Proteins; Proteomics; Spectroscopy;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780052
