Title :
Notice of Retraction
The Optimization of the Bio-Panning Process of the Affinity Ligand of Tyrosinase by Phage Display Technology
Author :
Wen Wang ; Hua-Li Nieu ; Tian-Xiang Chen ; Li-Min Zhu ; Branford-White, Christopher J.
Author_Institution :
Coll. of Chem., Chem. Eng. & Biotechnol., Donghua Univ., Shanghai, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The aim of this study was to search an optimal bio-panning process of phage display. In this study, the target protein is tyrosinase. Through changing the screening conditions, we found a successful method to obtain a high recovery ratio when the specificities of the sequences were not affected. Ultimately, the optimum bio-panning conditions are as follows: the incubate time is 40min, the wash time is 6 times and the wash time is 35min. The verifying experiment showed that this bio-panning process can obtain a high recovery ratio of phages, and the result of the ELISA showed that the specificities of the sequences were not affected.
Keywords :
biochemistry; bonds (chemical); enzymes; molecular biophysics; molecular configurations; optimisation; ELISA; affinity ligand; bio-panning process; phage display technology; screening conditions; sequences; target protein; time 35 min; time 40 min; tyrosinase; Chemicals; Cloning; Libraries; Materials; Peptides; Proteins; Skin;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780079
